Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PARP9 in samples. An antibody specific for PARP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PARP9-transfected cell lines displayed 4-fold higher cell migration compared to controls and suggested that PARP9 may promote the migration and dissemination of high-risk diffuse B-cell lymphomas. Semiquantitative RT-PCR of 28 primary diffuse large B-cell lymphomas detected significantly higher PARP9 expression in high-risk lymphomas compared to low-risk lymphomas. Northern blot analysis of human tissues detected strong expression in skeletal muscle, spleen, colon, peripheral blood lymphocytes, lymph node, and fetal liver, with lower expression in heart, placenta, lung, adult liver, kidney, pancreas, thymus, prostate, testis, ovary, and small intestine. Immunofluorescence and subcellular fractionation studies localized PARP9 to the nucleus in both transfected NIH3T3 cells and B lymphocytes.
